Part:BBa_K2050421
Thosea Asigna Virus 2A peptide linker (T2A)
2A peptide is a self-cleaving peptide linker. The sequence encoding the 2A peptide is typically included between the coding sequences of two proteins without stop codons to co-express the two proteins in a single vector. The two coding sequences will be transcribed onto one mRNA molecule along with the coding sequence for the 2A peptide. During translation, however, the peptide will cleave the peptide bond between the glycine and the proline residue on its C-terminus. The result is two different polypeptide chains.
The coding sequence for 2A peptide is far shorter than that of an IRES, making it preferable in constructing a multicistronic vector. The consequence of using the 2A peptide linker, however, is that the upstream protein will have additional residues from the 2A peptide on its C-terminus and the downstream protein will start with a proline.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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